Application of sewage sludge (SS) as a fertilizer contributes to the intensity of Zn, Ga, Y, Zr, Rh, Pb uptake by Jerusalem artichoke plants. Also, the translocation coefficients of Zn, Sr, Y, Rh, Pb vary in a wide range of values, depending on the level of use of SS. Jerusalem artichoke culture is characterized by a significant potential for biological absorption of Ni, Cu, Zn, Ga, Y, Nb and especially Rh, and the translocation coefficient of all studied heavy metals was ˃1. Jerusalem artichoke is characterized by certain features of intra-tissue pollution by heavy metals under the influence of SS application, which leads to an increase in this indicator compared to the application of mineral fertilizers in an equivalent dose of Zn, Y, Zr, as well as Ni, Cu, Ga, Sr, Nb, Pb in the highest doses (option 6). The use of SS composts with straw in the experiment leads to a significant decrease in intra-tissue pollution indicators compared to the application of fresh SS. Moreover, increasing the dose of compost from 20 t/ha to 30 t/ha contributes to the reduction of intra-tissue contamination of plants with Cu, Zn, Sr, Zr, Nb, Pb. The highest levels of the Integral indicator of vegetation cover pollution are determined by the application of fresh SS at the SS rate of 40 t/ha + N10P14K58. Jerusalem artichoke culture, given its significant potential for translocation of heavy metals, can be successfully used for phytoremediation of technogenically polluted areas and grown with the introduction of SS as fertilizers.