The main objective of this experiment was to determine the effectiveness of the organic compounds release from activated sludge flocs, due to the hydrodynamic disintegration conducted at different energy densities (140 ÷ 700 kJ/l). Indicators of the efficiency used for the process were the disintegration degree (DD) and the unit efficiency of organic compounds released (WCOD). Analysis of the results revealed that increasing the energy density resulted in an increase of concentration of soluble COD. The most intense release of organic compounds occurred when the energy density was increased from 140 to 420 kJ/l. Moreover, it was proved that by conducting disintegration using an energy density of 420 kJ/l results in a maximal efficiency of organic compound release (23.2 ÷ 30.0 mg ChZT/kJ).