Organic matter is a major component of soil. It is of considerable ecological importance given its role in determining soil health, influencing ecosystem productivity and climate. For this reason, it is essential to carry out studies to evaluate its dynamics in natural ecosystems. In this study, we aimed to explore the dynamics of soil organic matter (SOM) in forest ecosystems of the Central Plateau in Morocco, as well as to investigate the potential of spectral vegetation indices in modeling SOM. To this end, soil samples for analysis were collected from 30 sites across three vegetation types, including cork oak, Barbary thuja and scrub (matorral). In addition, the normalized difference vegetation index (NDVI) was extracted from Landsat 8 images to be used to model SOM using linear regression. Our results showed a weak although statistically significant (α < 0.05) correlation between NDVI and SOM at 0.45. In addition, only the scrub type showed a statistically significant (α < 0.05) relationship between its corresponding SOM and NDVI, and was therefore retained for modeling. Vegetation type had a statistically strong influence (α < 0.01) on SOM, with cork oak and garrigue ecosystems having the highest and lowest SOM contents with 5.61% and 2.36%, respectively. In addition, the highest SOM contents were observed under slightly acidic pH soils on mild, warm slopes at high altitude sites, while the lowest were found in lowland areas with predominantly weakly evolved soil.