The research activities carried out in the laboratory produce complex wastewater which most likely damage the environmental ecosystem if it is directly disposed of without treatment. Wastewater treatment in this study aimed to reduce the pollutant load contained in laboratory wastewater by using combination methods of Advanced Oxidation Processes (AOPs), and chemical-physical treatment namely coagulation and adsorption processes. The Photo-Fenton process was selected as one of AOPs applied in this treatment. The effect of molar ratio variation and irradiation time in the Photo-Fenton process on the pollution load in the form of pH, COD, BOD, TSS, and heavy metals Zn, Cu, Cr, Pb, and Fe was studied in this research. The results of the analysis of untreated laboratory wastewater samples showed that laboratory wastewater did not meet the wastewater quality standards regulated in the Regulation of the Minister of Environment of the Republic of Indonesia No. 5 of 2014 on Wastewater Quality Standards. In this study, laboratory wastewater was treated using the pre-treatment method of coagulation with alum and adsorption with activated carbon. The best results in this study were obtained in the final adsorption results after treatment with the Photo-Fenton method using a molar ratio of 1: 300 for 60 minutes in which several parameters such as pH, Zn, Cu, Pb, and Fe had met environmental quality standards with the value of each parameter of 7; 0.01 mg/L; 2.9 mg/L; 0.03 mg/L; and 3.15 mg/L respectively. Meanwhile, the percentage reduction of COD, BOD, and TSS parameters was 87.49%, 87.02%, and 72.45% respectively.