The maintenance of the human body, including any actions that may be taken to keep it free from disease and intoxication and to facilitate access to treatment, are all part of good health. Having a wide variety of molecular weights, polyethylene oxide [PEO] is a hydrophilic, uncrosslinked, nonlinear system polymer. It's made from ethylene oxide, which has a lot of advantages for medicine administration and antimicrobial purposes. Polyethylene oxide (PEO) bactericidal activity at different PEO concentrations value [80, 40, 20 and 10 μg/ml] against five isolates of Bacillus cereus isolated and identification from azo dye is investigated in this work (random selection from total isolates). The minimum inhibitory concentration [MIC] and minimum bactericidal concentration [MBC] of each isolate were calculated, and PEO's antibacterial activity was evaluated using the disk diffusion test. 85 Bacillus cereus isolates were collected from total azo dyes, PEO has a broad-spectrum antibacterial effect against tested bacteria, with an inverse connection between inhibitory zone diameter and PEO concentration, also even exceeds the activity of some drugs. The MICs of PEO ranged from 10 to 20 μg/ml, with MBCs ranging from 20 to 80 μg/ml. In other trials, PEO was shown to be strongly attached to bacterial cells, which might explain its effect on bacterial inhibitory growth and their invasion. At an appropriate concentration, PEO significantly inhibited bacterial growth. To avoid the development of antibiotic-resistant bacterial strains, it is strongly recommended that PEO be used as a cost-effective antibacterial agent, particularly when mixed with deys used at home or in enterprises.