The study aims to comparatively evaluate the performance of five bio-carrier media biochip, white 1, white 2, black and m-media 1 used in biological wastewater treatment systems. The objective is to determine the influence of media geometry and surface characteristics on biofilm formation, organic load removal, and overall treatment efficiency. A modified activated sludge system was used to assess the performance of each bio-media type. Key performance indicators such as chemical oxygen demand (COD), biochemical oxygen demand (BOD), total suspended solids (TSS), and biofilm biomass accumulation were measured. The analysis also incorporated literature based data to compare the tested media with conventional bio-carriers used in moving bed bioreactors (MBBR) and fixed film reactors. Among all tested media, white 1 media of size 12×25 mm demonstrated the highest biofilm biomass density (10.13 g/m²) and superior organic load handling, achieving the lowest COD (334.5 mg/L) and BOD (110.2 mg/L) values, with a maximum COD loading rate of 3.19 g/m². Biochip media exhibited the largest specific surface area (5500 m²/m³) and the highest biomass concentration per volume (6.10 kg/m³), though with comparatively lower specific loading rates. The M- media 1 of size 20×20 mm displayed moderate pollutant removal and limited biofilm accumulation despite a surface area of 950 m²/m³. The findings highlight that media geometry and surface characteristics significantly affect treatment performance. White 1 media 12×25 mm media proved most efficient for organic load removal. Optimized bio-media selection can enhance wastewater treatment efficiency and promote sustainable water reuse in agricultural and landscape irrigation applications.