This research aims to evaluate the biological insecticidal activity of the plant from the Asteraceae family Chrysanthemum (Glebionis segetum) on crop pest species: Thrips tabaci (thrips), Ditylenchus dipsaci (nematode), and Sitophilus oryzae (rice weevil). Materials and Methods: The flowers were dried in the shade, ground, then subjected to ultrasound-assisted extraction (40 kHz, 60 min, 50 °C) using a water/ethanol mixture design with a solid-liquid ratio of 1:10 (w/v). The obtained extracts were filtered, then lyophilized (72 h, 0.04–0.2 mbar). The tests were conducted according to a completely randomized design. Mortality was monitored over a period ranging from 1 to 144 hours. The results obtained show variable effectiveness depending on the extract and the target pest. Extract E5 showed strong effectiveness against both Thrips tabaci and Ditylenchus dipsaci, with mortality rates reaching (MR48h = 94.4%) and (MR48h = 90.0%), respectively, and corresponding LC50 values of 13.0 and 10.3 mg/mL. This extract also showed moderate activity against Sitophilus oryzae, while extracts E7 and E4 exhibited better activity against the latter, with mortalities of (MR48h = 70.8%) and (MR48h = 66.7%), respectively. In contrast, extract E2 showed a balanced activity spectrum, exceeding 50% mortality for each of the three pests, making it particularly suitable for crops affected by multiple pest species. Toxicological analyses allowed for the estimation of lethal concentrations (LC50), providing key parameters to compare the effectiveness of the extracts. conclusion: the exploration of ethanolic extracts from the wild plant Glebionis segetum (L) demonstrated significant potential underutilized for crop protection. However, as this study was limited to in vitro tests, field research is necessary to validate the efficacy of the extracts and identify the active compounds responsible for the observed effects.