The following study presents the effects of Ethylenediaminetetraacetic acid (EDTA), biochar, and humic acid on zinc and copper uptake in plant tissues. Treatments were conducted successively in an arrangement that the main consideration was towards metal uptake in root and shoot tissues by keeping the properties of chelation which is developed through EDTA and enhanced capabilities in the soil performance of biochar and humic acid. These results indicated that EDTA at 5 μg/kg significantly increased Zn accumulation in the roots at 861.361±1188.849 µg/kg, p=0.033, higher than that of both biochar and humic acid treatments combined. Among root tissues, Cu accumulation was also highest in EDTA treated at 2.5 µg/kg, amounting to 594.649±836.200 µg/kg, p=0.034. Most noticeably, humic acid at 10 μg/kg increased Cu uptake in the roots at 3109.425 ± 7187.589 μg/kg, p = 0.017, thus may have acted to increase metal availability. Shoot metal accumulation did not differ significantly across treatments, however. These results point to the effectiveness of EDTA in enhancing the root uptake of metals while biochar and humic acid generally produced small but differential effects. The findings underline the importance of choosing a treatment for metals uptake optimization in plants and enlighten the possible use of soil amendments for better nutrient management.