The paper deals with investigating the impact of heavy metals on biotic processes in the soils of the urban ecosystem of the city of Rivne. The territories of the northern part of the city, where the main industrial enterprises are concentrated, and the southern part of the city, with a significant impact of fugitive emissions from the railway and road transport, are characterized by the highest level of soil contamination. The soils are assigned to class III of hazard of contamination with heavy metals with "medium impact level" on biological processes, soil contamination category is "hazardous". The western and central parts of the city are marked by a significant level of contamination, the soils belong to class IV of hazard with "moderate impact level" on biological processes, the soil contamination category is "moderately hazardous". The eastern part of the city is the least contaminated and is assigned to class V of hazard. The contamination level is "low", the soil contamination category is "permissible".