Research into the formation of Pseudomonas savastanoi biofilms on olive trees is essential to prevent infections that induce tumor formation and damage tree health. To prevent the development of P. savastanoi biofilms, it is crucial to comprehend the factors influencing its adhesive behaviour. This research analysed the physicochemical properties of P. savastanoi in two types of media. P. savastanoi was cultivated in two media: nutrient agar (NA) and King B (KB), in solid and liquid forms. Wettability (θw), electron acceptor (γ+) / electron donor (γ-) properties, and surface free energy (ΔGiwi) are evaluated by contact angle measurements. The obtained results indicated that in solid media (NA and KB), P. savastanoi exhibited hydrophobic surface (θ_w^NA= 82.23°; θ_w^KB= 94.9°), with strong electron donor (γ_NA^-= 43.54 mJ.m-2; γ_KB^-= 58.34 mJ.m-2) and mild electron acceptor (γ_NA^+= 0.32 mJ.m-2; γ_KB^+= 0.52 mJ.m-2) properties, along with negative surface free energy (〖ΔG〗_iwi^NA = -76.13 mJ.m-2; 〖∆G〗_iwi^KB= -65.33 mJ.m-2). Conversely, in liquid media (NB and KB), the surface of P. savastanoi was generally hydrophilic (θ_w^NB= 55.43°; θ_w^KB= 64.43°), with strong electron donor (γ_NB^-= 29.23 mJ.m-2; γ_KB^-= 41.83 mJ.m-2) and a surface free energy that registers as positive. Modification of the growth medium composition conducted to minor variations in P. savastanoi hydrophobicity and surface free energy. By understanding the factors involved in this adhesive behaviour, we can develop ecological methods to protect crops and contribute to more efficient environmental management and conservation of natural resources.