The wastewater treatment process for middle rank coal employed coagulation-flocculation technology with bio coagulants to efficiently reduce the concentration of contaminants. This method utilized Moringa oleifera L. seed powder as the main bio coagulant, in addition to papaya seeds (Carica papaya Linn) and Ambon banana peel waste (Musa paradisiaca var) as secondary bio coagulants, which are combined with the main bio coagulant. The aim of this study was to determine the characteristics and effectiveness of bio coagulant compounds obtained from plants and waste materials. The study objective was also to investigate the effectiveness of combining bio-coagulants derived from Moringa oleifera L., Carica papaya, and Musa paradisiaca var in treating coal effluent. Biocoagulant is a natural alternative to chemicals like Aluminum Sulfate (Alum) that are detrimental to the environment. It is present in the ecosystem and serves as a substitute for these hazardous substances. The results revealed that the optimal dosage for combining bio coagulants was 1 gram per liter, with a composition ratio of 2 parts primary bio coagulant to 1 part auxiliary bio coagulant. The analysis of water pollutants showed a reduction in turbidity and Total Suspended Solid (TSS) by 99.26% and 99.11% respectively. Additionally, there was a decrease in the levels of heavy metals Iron (Fe) and Manganese (Mn) by 98.71% and 99.88% respectively.