Comparative Analysis of Phenolic and Flavonoid Content, Antioxidant, Antibacterial Activities, and Functional Groups of Chemicals from Hypericum perforatum L., and Papaver Rhoeas L. Flower Extracts
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Laboratory of Natural Resources and Sustainable Development, Faculty of Science, Ibn Tofail University, Morocco
Department of Plant and Environment Protection, National School of Agriculture, Meknes, Morocco
Laboratory of Biology and Health, Biology Department, Ibn Tofail University, Faculty of Sciences, Morocco.
Laboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement, Faculté des Sciences, Université Mohammed Premier, Morocco.
Laboratories TBC, Laboratory of Pharmacology, Pharmacokinetics and Clinical Pharmacy, Faculty of Pharmacy, University of Lille, France
Laboratory of Biological Engineering, Team of Functional and Pathological Biology, Faculty of Sciences and Technology, University Sultan Moulay Slimane, Morocco
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Morocco.
The Environment and Soil Microbiology Unit, Faculty of Sciences-Moulay Ismail University, Morocco
Corresponding author
Hajar Afqir   

Laboratory of Natural Resources and Sustainable Development, Faculty of Science, Ibn Tofail University, Morocco
Ecol. Eng. Environ. Technol. 2024; 2:88-101
In this study, we aimed to compare the phytochemical compounds (polyphenols and flavonoids), antioxidant activity, functional groups present in the compounds (FTIR), and anti-microbial effects, in aqueous and methanol extracts obtained from flowers of commercial Hypericum perforatum and native Papaver Rhoeas from Morocco. P. Rhoeas L was collected from El Lhaj Kaddour near Meknes, while H. perforatum L was bought in a dried state from a Moroccan herbalist in the same city. Total polyphenols were evaluated using the Folin-Ciocalteu reagent, respectively. The antioxidant activity was assessed via DPPH and anti-microbial effects were tested against six bacteria (Gram- and Gram+). In aqueous and methanol extracts of P. Rhoeas had the highest TPC value (23.67 ± 0.94 mg GAE/g; 15.86 ± 0.65 mg GAE/g) compared to H. perforatum (15.26 ± 1.30 mg GAE/g; 5.50 ± 1.13 GAE/g). The aqueous extract of Papaver Rhoeas exhibited the highest TFC at 14.36 ± 0.49 mg QE/g, while the methanol extract of Hypericum perforatum had the highest 10.65 ± 0.49 mg QE/g in TFC. In contrast, methanol and aqueous extracts of H. perforatum showed significant zones of inhibition against Staphylococcus aureus (9.5 ± 0.5 mm and 10.17 ± 0.29 mm) and Staphylococcus epidermidis (8.33 ± 0.58 mm and 9.33 ± 0.58 mm) respectively, with a minimum inhibitory concentration was estimated at 10 µL/ml. The FTIR analysis demonstrated that the extracts of both plants are rich in bioactive molecules with potential biological activities and a pharmaceutical industry perspective. Consequently, these Papaver Rhoeas and Hypericum perforatum extracts exhibit antioxidant and antibacterial activities.
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