These studies were designed to determine the optimum parameters of respirometric susceptibility test substrates for anaerobic digestion plant for example silage Sida. Biochemical dynamics of organic material determined by measuring blood OxiTop. The experiment was conducted in three series of research, different load measuring cells, the initial charge of organic compounds, a series of I – 5 gd.o.m/l, Series II – 10 gd.o.m/l, Series III – 20 gd.o.m/l. Fermentation was carried out in mesophilic conditions for 20 days in an anaerobic reaction chambers inoculated with activated sludge. Achieved a maximum of 3500 ml of biogas in series III, the least biogas created in Series I, which introduced the smallest load of organic compounds. Based on the results of the biogas production rate was determined in a series I was on y = 365 ml/gd.o.m, in the second series was y = 319 ml/gd.o.m. and the series III y = 278 ml/gd.o.m. Measuring the quality of the resulting biogas pointed to the relationship between the content of methane in the biogas with an initial charge of organic matter introduced into the reaction chamber, the methane content of the biogas ranged from 37% in the third series of up to 48% in the series and the experiment.