The paper presents possibility of using canola oil as active substance to fuel cell powering. For this purpose a prototype fuel cell was built. Cell was powered with canola oil emulsion. As a detergent Syntanol DS-10 was used. The mesh electrode with Pt catalyst was used as a anode, and mesh electrode with Ni-Co catalyst was used as a cathode. The measurements conducted in the temperature range 293-333K. The maximum current density reached the level of 2 mA/cm², and maximum power reached the level of 21 mW (at temp. 333K). So, the possibility of delivery of canola oil (in emulsion form) directly to the anode was shown. The obtain power is low, but was shown that it is possible to build the fuel cell powered with canola oil.