The distribution of zinc, nickel and chromium in the leaves and branches of three varieties of Salix viminalis differentiated fertilized was investigated. The biomass of Salix viminalis was harvested for investigation in the first and second year of application of mineral fertilizers and waste activate sludge in doses which contained 50, 150 and 200 kg of total nitrogen. The content of investigated metals was significantly differentiated upon the part of plant and fertilization. The amount of zinc, nickel and chromium were higher in leaves than in branches. The biomass of Salix viminalis contained the highest amount of zinc and the lowest of chromium. The highest content of zinc was determined in the biomass of Salix purpurea but lowest nickel and chromium in S. alba. The fertilization had differentiated influence upon the content of determined elements.