This article presents the results of studies of winter wheat species and varieties depending on the meteorological conditions of the research years. The purpose of the research is to determine the density of the productive stem, the mass of 1000 grains, the yield and quality of winter wheat grain, depending on the species and varietal composition. Methods. Experimental studies were conducted during 2015/2016-2020/2021 yrs in the experimental field of Mykolaiv National Agrarian University (Ukraine). Its predecessor was sown peas. Results. It was determined that a larger number of productive stems per unit area (467-853 PCs./ m2) formed plants of the studied varieties of soft winter wheat, while the smallest (443-694 PCs./ m2) were plants of winter durum wheat varieties. The largest mass of grain from one ear (1.06-1.20 g) on average over the years of research was formed by plants of spelt wheat varieties, and the smallest was formed 0.96-1.01 g of soft wheat plants. It was found that a higher yield of soft winter wheat (6.03 t/ha) on average over the years of research was formed in the Vidrada variety, hard wheat (5.58 t/ha) was formed in the Bosphorus variety, spelt was formed 5.36 t/ha in the Europa variety. The highest grain yield (7.13 t/ha) among the species and varieties put for study was provided by the Vidrada variety (T. aestivum) in 2016 yr, which was 5.3% more than in the variety Shestopalivka (T. aestivum), 5.9% more than the Linkor variety (T. durum), by 7.0% than the Bosphorus variety (T. durum), by 12.5% than that of the Europa variety (T. spelta) and 43.5% more than the Zorya Ukrainy variety (T. spelta). It was determined that the largest mass fraction of protein in grain was provided by the spelt Zorya Ukrainy wheat variety as 19.2-25.6% depending on the year of research, while the smallest was provided as 11.0-14.2% by the Shestopalivka soft wheat variety. In the conditions of the Southern steppe of Ukraine, to ensure a high grain yield (5.24-6.03 t/ha) with a high protein content (14.1-15.1%), it was recommended to sow the variety of soft winter wheat Vidrada, hard winter wheat as Linkor and spelt as Europe.