The present study aimed to evaluate the effects of human anthropic activities on the physicochemical and biological properties as well as the quantity of the groundwater in the central Rif of Morocco. Series of analyses were carried out on the water resources of this area. The interpretation of analytical data and the distribution of groundwater into groups were treated using multivariate statistical methods including Principal Component Analysis (PCA) and the Ascending Hierarchical Classification (CHA). The results of the present study showed strong mineralization of the investigated area waters. This study also indicated the impact of anthropogenic activities and their influences on the quality of groundwater in the central Rif, with the presence of total coliforms, fecal Escherichia coli type, and fecal streptococci intestinal enterococcal type, suggesting that the contamination of this groundwater was induced by human pollution. In addition, the infiltration of groundwater by wastewater from septic tanks, the use of wastewater for irrigation purposes, the increased use of fertilizers and pesticides in agriculture, and irregular rainfall in the region constitute the main factors of anthropogenic contamination of groundwater in the study area.